Author Archives: Grupo MVeIT

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Histological, cytogenetic and endocrine evaluation in twenty-five unilateral cryptorchid horses

Journal of Applied Animal Research pp. 1-4
Jose M. Vilar, Miguel Batista, Jose Maria Carrillo, Mónica Rubio, Joaquin Sopena & Desiree Álamo
Pages 1-4 | Received 21 Feb 2017, Accepted 17 May 2017, Published online: 30 May 2017

 

Data from 49 horses (25 unilateral cryptorchid and 24 as control group) are reported to determine macroscopic and microscopic characteristics of the testes, as well as to characterize their karyotype and hormonal levels. Histology showed that only Sertoli cells were found in the lumen of the seminiferous tubules of the cryptorchid testes, while spermatogenesis in the scrotal testes resulted normal. Cytogenetic evaluation showed that all cryptorchid horses were normal males (2n = 64, XY). In addition, the lower testosterone production observed in cryptorchid horses was associated to the smaller size of the retained testicle as well as to the lower number of Leydig cells adjacent to the seminiferous tubules. This study confirmed that a simple determination of plasma testosterone levels was enough to differentiate between non-breeding stallions, cryptorchid and castrated animals, avoiding the administration of hCG.

Artile

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Final outcome of raptors admitted to the Tafira Wildlife Rehabilitation Center, Gran Canaria Island, Spain (2003–2013)

Montesdeoca, N., Calabuig, P., Corbera, J. A., Rocha, J. & Orós, J.,

Animal Biodiversity and Conservation, 2017, 40.2: 211–220.

Abstract

Final outcome of raptors admitted to the Tafira Wildlife Rehabilitation Center, Gran Canaria Island, Spain (2003–2013).— The outcomes of wild raptors admitted to the Tafira Wildlife Rehabilitation Center in Gran Canaria Island, Spain, from 2003 to 2013 were analyzed using a quality auditing system based on the crude and stratified (by causes of admission) rates of four final outcome categories, time until death and length of stay as quality indicators. The outcome categories were: euthanasia (Er = 19.78%); unassisted mortality during hospitalization (Mr = 22.20%); release (Rr = 57.57%); and permanent captivity (Cr = 0.46%). Taking into account the particular vulnerability of insular raptor species and the high Rr achieved, findings from this study emphasize the importance of wildlife rehabilitation centers for the medical management of injured raptors and the subsequent release of rehabilitated individuals into the wild.

Web de Animal Biodiversity and Conservation

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A long-term retrospective study on rehabilitation of seabirds in Gran Canaria Island, Spain (2003-2013)

PLoS ONE 12(5): e0177366.

https://doi.org/10.1371/journal.pone.0177366

Natalia Montesdeoca1, Pascual Calabuig2, Juan A. Corbera1, Jorge Oros3*

1 Department of Animal Pathology, Veterinary Faculty, University of Las Palmas de Gran Canaria, Arucas,

Las Palmas, Spain, 2 Tafira Wildlife Rehabilitation Center, Cabildo de Gran Canaria, Tafira Baja, Las Palmas

de Gran Canaria, Spain, 3 Department of Morphology, Veterinary Faculty, University of Las Palmas de Gran

Canaria, Arucas, Las Palmas, Spain

Abstract

Aims

The aims of this study were to analyze the causes of morbidity and mortality in a large population of seabirds admitted to the Tafira Wildlife Rehabilitation Center (TWRC) in Gran Canaria Island, Spain, from 2003 to 2013, and to analyze the outcomes of the rehabilitation process.

Methods

We included 1,956 seabirds (133 dead on admission and 1,823 admitted alive) in this study. Causes of morbidity were classified into nine categories: light pollution (fallout), fishing gear interaction, crude oil, poisoning/intoxication, other traumas, metabolic/nutritional disorder, orphaned young birds, other causes, and unknown/undetermined. The crude and stratified (by causes of admission) rates of the three final disposition categories (euthanasia Er, unassisted mortality Mr, and release Rr), the time until death, and the length of stay were also studied for the seabirds admitted alive.

Results

Yellow-legged Gull (Larus michahellis) was the species most frequently admitted (46.52%), followed by Cory’s Shearwater (Calonectris diomedea borealis) (20.09%). The most frequent causes of morbidity were light pollution (fallout) (25.81%), poisoning/intoxication (24.69%), and other traumas (18.14%). The final disposition rates were: Er = 15.35%, Mr = 16.29%, and Rr = 68.34%. The highest Er was observed in the ‘other traumas’ category (58.08%). Seabirds admitted due to metabolic/nutritional disorder had the highest Mr (50%). The highest Rr was observed in the light pollution (fallout) category (99.20%).

Conclusions

This survey provides useful information for the conservation of several seabird species. We suggest that at least the stratified analysis by causes of admission of the three final disposition rates, and the parameters time until death and length of stay at the center should be included in the outcome research of the rehabilitation of seabirds. The high release rate for seabirds (68.34%) achieved at the TWRC emphasizes the importance of wildlife rehabilitation centers for the conservation of seabirds.


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Evaluation of pulmonary hypertension and clinical status in dogs with heartworm by Right Pulmonary Artery Distensibility Index and other echocardiographic parameters

Parasites and Vectors Open Access
Volume 10, Issue 1, 28 February 2017, Article number 106

Serrano-Parreño, B., Carretón, E., Caro-Vadillo, A., Falcón-Cordón, Y., Falcón-Cordón, S., Montoya-Alonso, J.A.

 

Abstract

Background: Pulmonary hypertension (PH) is a frequent and severe phenomenon in heartworm disease caused by Dirofilaria immitis, mainly caused by intimal proliferation of the arteries and pulmonary thromboembolisms. Transthoracic echocardiography is the method of choice for diagnosing PH in dogs although the diagnosis is often based on indirect and subjective parameters. The Right Pulmonary Artery Distensibility Index (RPAD Index) is a method that has been recently validated to estimate the presence and severity of PH in heartworminfected dogs. This study compared some echocardiographic parameters commonly used to estimate PH in 93 dogs infected by D. immitis and evaluated the impact of the parasite burden, microfilaremia, sex or origin of the dog (clientowned/shelter). Results: None of the studied echocardiographic variables seemed useful in the estimation of the evaluated clinical aspects, except for the PA/Ao ratio for parasite burden. The RPAD Index was determined in 88 of the dogs; of these, 70. 4% had PH (mild: 37.5%, moderate: 19.3%, severe: 13.6%). This Index showed nonsignificant differences according to microfilaremia, sex, origin or parasite burden. Symptomatic dogs showed PH more often and displayed more severe PH, in addition the presence of symptoms was greater among dogs with high burden; on the other hand 64.4% of asymptomatic dogs had some degree of PH according to the RPAD Index. Apart from the PA/Ao ratio, the other evaluated echocardiographic variables were not useful in evaluating of the hypertensive status of the heartworminfected dog compared to the RPAD Index. Conclusions: The estimation of most common indirect parameters is not useful in predicting PH in heartworminfected dogs. The results confirm the RPAD Index as an objective and supportive test in the monitoring and evaluation of PH in the heartworminfected dog, and show a potential diagnostic value for the detection of PH in asymptomatic animals.

Articulo

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Static posturography: A new perspective in the assessment of lameness in a canine model

Volume 12, Issue 1, January 2017, Article number e0170692
Open Access
Manera, M.E., Carrillo, J.M., Batista, M., Rubio, M., Sopena, J., Santana, A., Vilar, J.M.
a Departamento de Patología Animal, Instituto Universitario de Investigaciones Biomédicas Y Sanitarias, Universidad de Las Palmas de Gran Canaria, Arucas, Las Palmas, Spain
b Departamento Medicina Y Cirugía Animal, Cátedra García Cugat, Universidad CEU Cardenal Herrera, Valencia, Spain
c Departamento de Matemáticas, Universidad de Las Palmas de Gran Canaria, Las Palmas, Spain.

Abstract

The aim of this study was to assess the static posturography in dogs as a useful tool for diagnosis of lameness by means of the use of a pressure platform. For this purpose, a series of different parameters (pressure distribution, area of support, mean pressure, maximum pressure and statokinesiograms) were obtained from five lame dogs with unilateral elbow osteoarthritis treated with plasma rich in growth factors. Data were obtained before and 3 months after treatment, and results were compared with a control group of sound dogs of similar conformation. Significant differences were found in the above mentioned parameters between sound and lame limbs. Improvement after 3 months of treatment was also detected, demonstrating that this multi-parametric technique is an effective and reliable method for the assessment of lameness in dogs. © 2017 Manera et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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Serological survey of antibodies to Toxoplasma gondii and Neospora caninium in goat population in Canary Islands (Macaronesia Archipelago, Spain)

Volume 147, February 2017, Pages 73–76

E. Rodríguez-Ponce, 1, M. Conde, J.A. Corbera, J.R. Jaber , M.R. Ventura, C. Gutiérrez
a Unit of Animal Parasitology, Faculty of Veterinary Sciences, University of Las Palmas de Gran Canaria, Las Palmas, Canary Islands, Spain
b Research Institute of Biomedicine and Health Sciences, University of Las Palmas de Gran Canaria, Las Palmas, Canary Islands, Spain
c Department of Morphology, Faculty of Veterinary Sciences, University of Las Palmas de Gran Canaria, Las Palmas, Canary Islands, Spain
d Unit of Animal Production, Faculty of Veterinary Sciences, University of Las Palmas de Gran Canaria, Las Palmas, Canary Islands, Spain

Abstract

Toxoplasma gondii and Neospora caninum are protozoan parasites with worldwide distribution affecting different species. Previous studies performed in Gran Canaria Island on T. gondii revealed 63.3% of seroprevalence for both, humans and goats. Therefore, in order to evaluate the current status of both parasites in goats in the Canary Islands, 552 animals located in different climate zones were randomly selected. Sera were analyzed using specific enzyme linked immunosorbent assay (ELISA), revealing an overall seroprevalence of 7.8% and 1.08% for T. gondii and N. caninum, respectively. Prevalence in individual islands ranged from 0% to 30% for T. gondii and from 0% to 3.3% for N. caninum. This is the first epidemiological study done in the Canary Islands, and it revealed that the distribution and prevalence of these parasites were related to the different climates present in each island. Compared to the previous studies, this survey demonstrated a diminished seroprevalence for toxoplasmosis in one of the seven islands studied.


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Pulmonary hypertension in dogs with heartworm before and after the adulticide protocol recommended by the American Heartworm Society

Volume 236, 15 March 2017, Pages 34-37
Serrano-Parreño, B.a, Carretón, E.a , Caro-Vadillo, A.b, Falcón-Cordón, S.a, Falcón-Cordón, Y.a, Montoya-Alonso, J.A.a
a Internal Medicine, Faculty of Veterinary Medicine, Research Institute of Biomedical and Health Sciences (IUIBS), University of Las Palmas de Gran Canaria, Las Palmas de Gran Canaria, Spain
b Department of Animal Medicine and Surgery, Veterinary Faculty, Complutense University of Madrid, Madrid, Spain.

Abstract

Pulmonary hypertension (pH) is a frequent and severe phenomenon in heartworm disease (Dirofilaria immitis). There is a lack of studies assessing the evolution of the proliferative endarteritis and pH caused by D. immitis after the death of the parasites, so this study evaluated the influence that the elimination of the worms exerts over the pulmonary pressure and therefore evolution of the endarteritis, through the evaluation of the Right Pulmonary Artery Distensibility (RPAD) Index and other echocardiographic measurements in 2D mode, M-mode and Doppler echocardiography in 34 dogs naturally infected by D. immitis on day 0, and one month after the last adulticide dose (day 120). pH, based on the determination of the RPAD Index, was present in 68% of the dogs (n = 23) on day 0 and on day 120. No significant differences were observed between the RPAD Index between the two measurements, and only significant differences were found in pulmonary deceleration time, ejection time, and left ventricular internal diameter in telediastole when measurements from day 0 and day 120 were compared. There was not any worsening in the development of pH after the elimination of the parasites, independently of the parasite burden. During the adulticide treatment, the death of the worms causes thromboembolism and tends to worsen the vascular damage and presence of pH. It seems that following the adulticide protocol recommended by the American Heartworm Society with the previous elimination of Wolbachia and reduction of microfilariae followed by the stepped death of the worms did not cause a significant aggravation of the pulmonary damage of the treated dogs. Neither is present any significant improvement in the RPAD Index on day 120; probably, more time is needed before appreciating some positive changes after the elimination of the worms and Wolbachia from the vasculature and further studies are necessary.

Link to the DOI

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The heartworm (Dirofilaria immitis) seems to be able to metabolize organochlorine pesticides and polychlorinated biphenyls: A case-control study in dogs

September 05, 2016

Henríquez-Hernández, L.A.a, Carretón, E.c, Camacho, M.a, Montoya-Alonso, J.A.c,  Boada, L.D.ab, Valerón, P.F.ab, Falcón-Cordón, Y.c, Falcón-Cordón, S.c, Almeida-González, M.a, Zumbado, M.a, Luzardo, O.P.ab

Toxicology Unit, Research Institute of Biomedical and Health Sciences (IUIBS), Universidad de Las Palmas de Gran Canaria, Paseo Blas Cabrera Felipe s/n, 35016, Las Palmas de Gran Canaria, Spain
Spanish Biomedical Research Centre in Physiopathology of Obesity and Nutrition (CIBERObn), Paseo Blas Cabrera Felipe s/n, 35016, Las Palmas de Gran Canaria, Spain
Internal Medicine Service, Faculty of Veterinary Medicine, Research Institute of Biomedical and Health Sciences (IUIBS), Universidad de Las Palmas de Gran Canaria, Paseo Blas Cabrera Felipe s/n, 35016, Las Palmas de Gran Canaria, Spain

Abstract

It has been described that the co-existence of parasite infection and chemical exposure has various effects on the accumulation of persistent organochlorine pollutants (POPs) in the host. Certain parasites are not only able to accumulate POPs but also seem to have the ability to metabolize certain compounds. We have designed a case-control study aimed to disclose the role of . Dirofilaria immitis in the bioavailability of POPs in dogs trying to know whether these parasites store or metabolize the POPs. A total of 40 common POPs (18 polychlorinated biphenyls congeners (PCBs) and 22 organochlorine pesticides were quantified in dog serum. The study included three groups of dogs prospectively recruited in the island of Gran Canaria (Canary Islands, Spain): a) control animals, non-parasitized (serologically tested negative, . n = 24); b) a group constituted by dogs tested positive for heartworm disease (D. immitis) and negative for other parasites (n = 25); and c) the same group of parasitized dogs after the treatment against the parasite (n = 25). The presence of . D. immitis was strongly associated with lower serum levels of a wide range of pollutant in their hosts (PCB congeners 28, 105, 118, 123, 138, 153, 167 and 180; hexachlorobenzene, lindane, aldrin, dieldrin, and methoxychlor). The serum levels of these substances remained at very low levels after the treatment against the parasite, suggesting that . D. immitis do not simply store such compounds, but they probably have some ability to metabolize these pollutants. We encourage the use of the parasite infestation status as a cofactor that needs to be taken into account in studies aimed to evaluate the serum levels of POPs.

Artículo

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Effect of intraarticular inoculation of mesenchymal stem cells in dogs with hip osteoarthritis by means of objective force platform gait analysis: Concordance with numeric subjective scoring scales  

Volume 12, Issue 1, 7 October 2016, Article number 223
Vilar, J.M.a , Cuervo, B.b , Rubio, M.b , Sopena, J.b , Domínguez, J.M.c , Santana, A.a , Carrillo, J.M.b  

Universidad de Las Palmas de Gran Canaria, Department of Animal Pathology, Trasmontaña S/N, Las Palmas, Arucas, Spain
Cátedra Garcia Cugat, CEU Cardenal Herrera University, Department of Animal Medicine and Surgery, C/Tirant lo Blanc, 7, Valencia, Alfara del Patriarca, Spain
University of Cordoba, Department of Animal Medicine and Surgery, Cordoba, Spain

Abstract

Background: Subjective pain assessment scales have been widely used for assessing lameness in response to pain, but the accuracy of these scales has been questioned. To assess scale accuracy, 10 lame, presa Canario dogs with osteoarthritis (OA) associated with bilateral hip dysplasia were first treated with mesenchymal stem cells. Then, potential lameness improvement was analyzed using two pain scales (Bioarth and visual analog scale). These data were compared with similar data collected using a force platform with the same animals during a period of 6 months after treatment. Results: The F test for intraclass correlation showed that concordance in pain/lameness scores between the 2 measuring methodologies was not significant (P value ≥ 0.9213; 95 % confidence interval, -0.56, 0.11). Although subjective pain assessment showed improvement after 6 months, force platform data demonstrated those same animals had returned to the initial lameness state. Conclusion: Use of pain assessment scales to measure lameness associated with OA did not have great accuracy and concordance when compared with quantitative force platform gait analysis.

Artículo

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Influence of different anaesthetic protocols over the sperm quality on the fresh, chilled (4°C) and frozen-thawed epididymal sperm samples in domestic dogs

Volume 51, Issue 5, 1 October 2016, Pages 758-765

Batista, M. ,Vilar, J., Rosario, I., Terradas, E.

Unit of Reproduction, Universitary Institute of Biomedical Research and Health, University of Las Palmas de Gran Canaria, Arucas, Las Palmas, Spain

Abstract

This study assessed the influence of three different anaesthetic protocols on semen quality obtained from the epididymis. Sixty male dogs undergoing to routine sterilization were assigned to three anaesthetic protocols: thiopental group (TG, n = 20), propofol group (PG, n = 20) and ketamine–dexmedetomidine group (KDG, n = 20). Immediately after orchidectomy, the cauda epididymides and vas deferent ducts were isolated and then a retrograde flushing was performed to collect spermatozoa. In experiment 1, after the initial evaluation of the semen (sperm concentration, sperm motility and the percentages of live spermatozoa, abnormal spermatozoa and acrosome membrane integrity), semen samples were diluted in Tris-glucose-egg yolk extender and chilled for 48 hr, and the sperm motility was assessed at 6, 24 and 48 hr. In experiment 2, semen samples were diluted in Tris-glucose-egg yolk extender and chilled for 24 hr, and then samples were frozen in two extenders with different glycerol concentrations, to reach a final concentration of 50–100 × 106 spermatozoa ml−1, 20% egg yolk, 0.5% Equex and 4% and 5% glycerol, respectively. Mean values of total sperm concentration, sperm viability and the percentages of intact acrosome and abnormal spermatozoa were not significantly different between experimental groups, and therefore, the anaesthetic protocols assessed did not affect sperm parameters mentioned above. However, our study confirmed a detrimental effect of the use of thiopental (TG) over the total sperm motility (p < 0.05) and progressive sperm motility (p < 0.05) of the fresh and chilled epididymal sperm samples. The anaesthetic protocols including the application of propofol or ketamine–dexmedetomidine can be used to recover sperm in domestic canids without significant changes in sperm quality compared when semen is collected routinely and these techniques could be applicable to endangered wild canids.