{"id":388,"date":"2014-09-06T17:08:55","date_gmt":"2014-09-06T17:08:55","guid":{"rendered":"http:\/\/medicinaveterinaria.ulpgc.es\/?p=388"},"modified":"2016-05-29T17:15:31","modified_gmt":"2016-05-29T17:15:31","slug":"rectal-pre-treatment-with-ozonized-oxygen-o3-aggravates-clinic-status-in-septic-rats-treated-with-amoxicillinclavulanate","status":"publish","type":"post","link":"https:\/\/medicinaveterinaria.ulpgc.es\/?p=388","title":{"rendered":"Rectal pre-treatment with ozonized oxygen (O3) aggravates clinic status in septic rats treated with amoxicillin\/clavulanate"},"content":{"rendered":"<p><strong>Enfermedades Infecciosas y Microbiologia Clinica Monografias.\u00a0<\/strong>Volume 33, Issue 7, 2015, Pages 469-475<br \/>\n<em>Mart\u00edn-Barrasa, J.L. , Cordovez, C.M.b De Los Monteros Y Zayas, A.E., D Santa Ana, M.C.J., Varas, B.C., Thomas, P.H., Benitez, A.B., Montoya-Alonso, J.A., Garc\u00eda-Bello, M., Campelo, F.A.f Tejedor-Junco, M.T.<\/em><\/p>\n<h2 class=\"subTitle paddingT7 paddingB10\">Abstract<\/h2>\n<p id=\"recordAbs\" class=\"marginB3\" align=\"justify\">Introduction: Despite the advanced antibiotic therapies, sepsis continues being a clinical entity with high morbidity and mortality. The ozone\/oxygen mixture (O3\/O2) has been reported to exhibit positive effects on immunity. The aim of our study was to analyze whether (O3\/O2) combined with amoxicillin\/ clavulanate has any influence on the morbidity and mortality of septic rats. Methods: We used 48 Sprague-Dawley rats randomly allocated to 6 groups (n = 8): healthy (C), septic (I), healthy + ozone therapy (O3), septic + amoxicillin\/clavulanate (AMC), septic + amoxicillin\/clavulanate + ozone therapy (AMC\/O3) and septic + ozone therapy (I\/O3). O3\/O2 was administered rectally at increasing O3concentrations during 10 days prior to the onset of sepsis model (intraperitoneally injection of fecal material) or saline administration in healthy control rats. Later (post-inoculation), 3 days per week, O3was also administered. Vital signs were recorded, and microbiological, hematological and histopathological studies were performed. Results: The number of surviving animal\/total was higher in AMC (8\/8) than in AMC\/O3 (4\/8) p = 0.077. The percentage of surviving animals with pneumonia was higher in AMC\/O3 than in AMC (100% vs 37.5%). In dead animals, AMC\/O3 rats had a significantly higher percentage of lesions: Cardiac lesions, pulmonary hemorrhages and pleuritis (100%) and serositis\/peritonitis (75%). Only Escherichia coli (2 different biotypes) was isolated from blood and\/or peritoneal fluid from all infected groups. A significant decrease in the percentage of band neutrophils from the surviviors belonging to AMC\/O3 vs AMC was observed (p 0.05). Conclusion: Rectal pre-treatment with O3\/O2 aggravates clinic status in septic rats treated with amoxicillin\/ clavulanate.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Enfermedades Infecciosas y Microbiologia Clinica Monografias.\u00a0Volume 33, Issue 7, 2015, Pages 469-475 Mart\u00edn-Barrasa, J.L. , Cordovez, C.M.b De Los Monteros Y Zayas, A.E., D Santa Ana, M.C.J., Varas, B.C., Thomas, P.H., Benitez, A.B., Montoya-Alonso, J.A., Garc\u00eda-Bello, M., Campelo, F.A.f Tejedor-Junco, M.T. Abstract Introduction: Despite the advanced antibiotic therapies, sepsis continues being a clinical entity with<\/p>\n","protected":false},"author":1,"featured_media":390,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[16],"tags":[],"_links":{"self":[{"href":"https:\/\/medicinaveterinaria.ulpgc.es\/index.php?rest_route=\/wp\/v2\/posts\/388"}],"collection":[{"href":"https:\/\/medicinaveterinaria.ulpgc.es\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/medicinaveterinaria.ulpgc.es\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/medicinaveterinaria.ulpgc.es\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/medicinaveterinaria.ulpgc.es\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=388"}],"version-history":[{"count":1,"href":"https:\/\/medicinaveterinaria.ulpgc.es\/index.php?rest_route=\/wp\/v2\/posts\/388\/revisions"}],"predecessor-version":[{"id":389,"href":"https:\/\/medicinaveterinaria.ulpgc.es\/index.php?rest_route=\/wp\/v2\/posts\/388\/revisions\/389"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/medicinaveterinaria.ulpgc.es\/index.php?rest_route=\/wp\/v2\/media\/390"}],"wp:attachment":[{"href":"https:\/\/medicinaveterinaria.ulpgc.es\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=388"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/medicinaveterinaria.ulpgc.es\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=388"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/medicinaveterinaria.ulpgc.es\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=388"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}