{"id":461,"date":"2013-10-01T18:46:54","date_gmt":"2013-10-01T18:46:54","guid":{"rendered":"http:\/\/medicinaveterinaria.ulpgc.es\/?p=461"},"modified":"2016-05-29T18:49:48","modified_gmt":"2016-05-29T18:49:48","slug":"middle-east-respiratory-syndrome-coronavirus-neutralising-serum-antibodies-in-dromedary-camels-a-comparative-serological-study-article","status":"publish","type":"post","link":"https:\/\/medicinaveterinaria.ulpgc.es\/?p=461","title":{"rendered":"Middle East respiratory syndrome coronavirus neutralising serum antibodies in dromedary camels: A comparative serological study (Article)"},"content":{"rendered":"<p><strong>The Lancet Infectious Diseases<\/strong><br \/>\nVolume 13, Issue 10, October 2013, Pages 859-866<br \/>\n<em>Reusken, C.B.E.M. , Haagmans, B.L., M\u00fcller, M.A., Gutierrez, C., Godeke, G.-J., Meyer, B., Muth, D., Raj, V.S., Vries, L.S.D., Corman, V.M., Drexler, J.-F., Smits, S.L., El Tahir, Y.E., De Sousa, R., van Beek, J., Nowotny, N., van Maanen, K., Hidalgo-Hermoso, E., Bosch, B.-J., Rottier, P., Osterhaus, A., Gort\u00e1zar-Schmidt, C., Drosten, C., Koopmans, M.P.G.<\/em><\/p>\n<h2 class=\"subTitle paddingT7 paddingB10\">Abstract<\/h2>\n<p id=\"recordAbs\" class=\"marginB3\" align=\"justify\">Background: A new betacoronavirus-Middle East respiratory syndrome coronavirus (MERS-CoV)-has been identified in patients with severe acute respiratory infection. Although related viruses infect bats, molecular clock analyses have been unable to identify direct ancestors of MERS-CoV. Anecdotal exposure histories suggest that patients had been in contact with dromedary camels or goats. We investigated possible animal reservoirs of MERS-CoV by assessing specific serum antibodies in livestock. Methods: We took sera from animals in the Middle East (Oman) and from elsewhere (Spain, Netherlands, Chile). Cattle (n=80), sheep (n=40), goats (n=40), dromedary camels (n=155), and various other camelid species (n=34) were tested for specific serum IgG by protein microarray using the receptor-binding S1 subunits of spike proteins of MERS-CoV, severe acute respiratory syndrome coronavirus, and human coronavirus OC43. Results were confirmed by virus neutralisation tests for MERS-CoV and bovine coronavirus. Findings: 50 of 50 (100%) sera from Omani camels and 15 of 105 (14%) from Spanish camels had protein-specific antibodies against MERS-CoV spike. Sera from European sheep, goats, cattle, and other camelids had no such antibodies. MERS-CoV neutralising antibody titres varied between 1\/320 and 1\/2560 for the Omani camel sera and between 1\/20 and 1\/320 for the Spanish camel sera. There was no evidence for cross-neutralisation by bovine coronavirus antibodies. Interpretation: MERS-CoV or a related virus has infected camel populations. Both titres and seroprevalences in sera from different locations in Oman suggest widespread infection. Funding: European Union, European Centre For Disease Prevention and Control, Deutsche Forschungsgemeinschaft.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The Lancet Infectious Diseases Volume 13, Issue 10, October 2013, Pages 859-866 Reusken, C.B.E.M. , Haagmans, B.L., M\u00fcller, M.A., Gutierrez, C., Godeke, G.-J., Meyer, B., Muth, D., Raj, V.S., Vries, L.S.D., Corman, V.M., Drexler, J.-F., Smits, S.L., El Tahir, Y.E., De Sousa, R., van Beek, J., Nowotny, N., van Maanen, K., Hidalgo-Hermoso, E., Bosch, B.-J.,<\/p>\n","protected":false},"author":1,"featured_media":463,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[4,11],"tags":[],"_links":{"self":[{"href":"https:\/\/medicinaveterinaria.ulpgc.es\/index.php?rest_route=\/wp\/v2\/posts\/461"}],"collection":[{"href":"https:\/\/medicinaveterinaria.ulpgc.es\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/medicinaveterinaria.ulpgc.es\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/medicinaveterinaria.ulpgc.es\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/medicinaveterinaria.ulpgc.es\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=461"}],"version-history":[{"count":1,"href":"https:\/\/medicinaveterinaria.ulpgc.es\/index.php?rest_route=\/wp\/v2\/posts\/461\/revisions"}],"predecessor-version":[{"id":462,"href":"https:\/\/medicinaveterinaria.ulpgc.es\/index.php?rest_route=\/wp\/v2\/posts\/461\/revisions\/462"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/medicinaveterinaria.ulpgc.es\/index.php?rest_route=\/wp\/v2\/media\/463"}],"wp:attachment":[{"href":"https:\/\/medicinaveterinaria.ulpgc.es\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=461"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/medicinaveterinaria.ulpgc.es\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=461"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/medicinaveterinaria.ulpgc.es\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=461"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}