Grupo MVeIT – Grupo de Investigación del Instituto Universitario de Investigaciones Biomédicas y Sanitarias (IUIBS) de la Universidad de Las Palmas de Gran Canaria.

26
Jun 18
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Doxycycline treatment for Dirofilaria immitis in dogs: impact on Staphylococcus aureus and Enterococcus antimicrobial resistance

Veterinary Research Communications 24 June 2018, Pages 1-6

Tejedor-Junco, M.T.^a, González-Martín, M.^a, Bermeo-Garrido, E.^b, Villasana-Loaiza, R.^b, Carretón-Gómez, E.^a

^aResearch Institute of Biomedical and Health Sciences, University of Las Palmas de Gran Canaria, Paseo Blas Cabrera Felipe “Físico” s/n, Las Palmas de Gran Canaria, Canary Islands, Spain
^bMicrobiology, Faculty of Veterinary, University of Las Palmas de Gran Canaria, P.O. BOX 550, Las Palmas de Gran Canaria, Canary Islands, Spain

Abstract

Doxycycline is an antibiotic that, in addition to the classic antibacterial use, is also prescribed to fight parasitic diseases, like heartworm disease in dogs. Despite the concern that the overuse of this antibiotic may decrease susceptibility of clinically important bacteria, the consequences of the prolonged doxycycline therapy in heartworm-infected dogs have never been studied before. We have analyzed the impact of this therapy on Staphylococcus aureus and Enterococcus antimicrobial resistance. In this study, 17 heartworm-infected dogs (10 that had completed the doxycycline treatment and 7 dogs that had not yet begun) were included. Twenty-four isolates of Staphylococcus aureus were obtained from two locations of each dog. After treatment, 73.3% of isolates were resistant to at least one antibiotic but only 22.2% of isolates before treatment. Most of doxycycline resistant isolates were obtained from dogs that have received treatment. Erythromycin resistance or intermediate susceptibility was detected in 45.6% of isolates, most of them from dogs after treatment. For Enterococci, 48 isolates were obtained from fecal samples (25 before treatment and 23 after treatment). Before treatment, 32% of isolates were resistant at least to one antibiotic while after, this data increase up to 65%. Comparing isolates before and after treatment, a clear increase in resistance to doxycycline (12% against 21.74%) and erythromycin (20% against 39.13%) was observed. Although the present work is a preliminary research, the results encourages the development of further studies to determinate the effect of prolonged doxycycline therapy on antimicrobial resistance.

04
Abr 18
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3-D computed tomography reconstruction: Another tool to teach anatomy in the veterinary colleges

Iranian Journal of Veterinary ResearchVolume 19, Issue 1, 2018, Pages 1-2

3-D computed tomography reconstruction: Another tool to teach anatomy in the veterinary colleges(Letter)

Jaber, J.R. Email Author, Carrascosa, C. ,Arencibia, A., Corbera, J.A. , Ramirez, A.S. Melian, C.

Article in PDF

24
Mar 18
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Posturography and dynamic pedobarography in lame dogs with elbow dysplasia and cranial cruciate ligament rupture

BMC Veterinary ResearchOpen AccessVolume 14, Issue 1, 24 March 2018, Article number 108

Posturography and dynamic pedobarography in lame dogs with elbow dysplasia and cranial cruciate ligament rupture(Article)

Carrillo, J.M.^a, Manera, M.E.^b, Rubio, M.^a, Sopena, J.^a Email Author, Santana, A.c, Vilar, J.M.^b

^aUniversidad CEU Cardenal Herrera, Departamento Medicina y Cirugía Animal, Cátedra García Cugat, Valencia, Spain
^bUniversidad de las Palmas de Gran Canaria, Departamento de Patología Animal, Instituto Universitario de Investigaciones Biomédicas y Sanitarias, Arucas, Las Palmas, Spain
^cUniversidad de las Palmas de Gran Canaria, Departamento de Matemáticas, Las Palmas, Spain

Abstract

Background: The usefulness of studying posture and its modifications due to locomotor deficiencies of multiple origins has been widely proven in humans. To assess its suitability in the canine species, static posturography and dynamic pedobarography were performed on lame dogs affected with unilateral elbow dysplasia and cranial cruciate ligament rupture by using a pressure platform. With this objective, statokinesiograms and stabilograms, the percentage of pressure distribution between limbs, paw area, mean pressure, and peak pressure, were obtained from lame and sound dogs. These data were compared with Peak Vertical Force values originated from a force platform in the same recording sessions. Results: Significant differences were found in the parameters mentioned above between sound and lame dogs and limbs. Conclusions: Posturography and pedobarography are useful and reliable for the monitoring of fore and hindlimb lameness in dogs, providing a new set of parameters for lameness detection.

Article in PDF

03
Mar 18
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Effect of leukocyte-reduced platelet-rich plasma on osteoarthritis caused by cranial cruciate ligament rupture: A canine gait analysis model

PLoS ONEOpen AccessVolume 13, Issue 3, March 2018, Article number e0194752

Effect of leukocyte-reduced platelet-rich plasma on osteoarthritis caused by cranial cruciate ligament rupture: A canine gait analysis model(Article)(Open Access)

Vilar, J.M.^a^b Manera, M.E.^a, Santana, A.^a, Spinella, G.^c, Rodriguez, O.^a, Rubio, M.^d, Carrillo, J.M.^d, Sopena, J.^d, Batista, M.^a^b

^aDepartamento de Patología Animal, Facultad de Veterinaria de la Universidad de Las Palmas de Gran Canaria, Trasmontaña S/N, Arucas, Las Palmas, Spain

^bInstituto Universitario de Investigaciones Biomédicas y Sanitarias, Universidad de Las Palmas de Gran Canaria, Juan de Quesada 30, Las Palmas de Gran Canaria, Las Palmas, Spain
^cDipartimento di Science Mediche Veterinarie, Facoltà di Veterinaria de la Università di Bologna, Via Tolara di sopra 50, Ozzano, Bologna, Italy

Abstract

The goal of this study was to objectively assess the effect of a platelet-rich plasma (PRP) derivate in English bulldogs with stifle degenerative joint disease secondary to cranial cruciate ligament rupture (CCLR). We used a force platform and affixed electrogoniometers to measure peak vertical force (PVF), vertical impulse (VI), stance time (ST), and angular range of motion (AROM), from 12 lame client-owned English bulldogs with post-CCLR stifle joint abnormalities. The 12 affected subjects were treated with 4 intra-articular injections of PRP, at 30-day intervals. Ten untreated, sound English bulldogs were used as a reference group. Clinical outcomes were evaluated using a linear mixed effects model. Mean values of PVF, VI, ST, and AROM were improved within the first 3 months post-treatment in the CCLR group, with mean measured changes increasing to maximum 4.56% body weight gain, 1.5% body weight/second, 0.07 seconds, and 6.18 degrees, respectively. The effects declined progressively after the treatment interval, ending at nearly initial levels after 6 months. This study demonstrates that dogs with CCLR treated with intra-articular PRP had improved PVF, VI, ST, and AROM over time; the duration of effect was waning by the end of the post-treatment period.

02
Feb 18
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The Canary Islands as a model of risk of pulmonary dirofilariasis in a hyperendemic area

Parasitology ResearchVolume 117, Issue 3, 1 March 2018, Pages 933-936

The Canary Islands as a model of risk of pulmonary dirofilariasis in a hyperendemic area.

Cabrera, E.D., Carretón, E., Morchón, R., Falcón-Cordón, Y., Falcón-Cordón, S., Simón, F., Montoya-Alonso, J.A

Abstract

We assessed the risk of human pulmonary dirofilariasis in the Canary Islands, hyperendemic for canine heartworm (Dirofilaria immitis), a zoonotic vector-borne infection. For this purpose, 1479 inhabitants were tested for anti-D. immitis antibodies. Four of the 7 islands presented high seroprevalences (from 6.2 to 12.7%), therefore high exposure to the parasite and risk of zoonotic infection. These corresponded to those islands with high canine prevalences of dirofilariasis and favourable climatic conditions for the development of mosquito vectors. The lowest prevalences (from 0 to 1.6%) were found in the desert islands and those with low canine prevalences of heartworm. Seroprevalences were very variable inside each island as well, being related to the climate and demographic factors. Human pulmonary dirofilariasis is an emerging zoonosis worldwide which frequently goes undiagnosed. Serological studies could be useful for the correct evaluation of the risk of infection among the human population, and study of the health implications of the continuous contact with the parasite in endemic areas. Sanitary authorities should be aware of the current epidemiological data, and physicians should include human dirofilariasis in the differential diagnosis of pulmonary nodules.

20
Dic 17
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Causes of morbidity and mortality, and rehabilitation outcomes of birds in Gran Canaria Island, Spain

BIRD STUDY, 2017

https://doi.org/10.1080/00063657.2017.1411464

Natalia Montesdeoca, Pascual Calabuig, Juan A. Corbera, John E. Cooper & Jorge Orós

a Veterinary Faculty, University of Las Palmas de Gran Canaria, Arucas Las Palmas, Spain; b Tafira Wildlife Rehabilitation Centre (Cabildo de Gran Canaria), Las Palmas de Gran Canaria, Spain; c Durrell Institute of Conservation and Ecology, University of Kent, Canterbury, UK

Abstract

Capsule: Anthropogenic factors were identified as the main threats to wild birds in Gran Canaria.

Aims: To analyse the causes of morbidity and mortality in a large sample of selected orders of birds admitted to the Tafira Wildlife Rehabilitation Centre (TWRC) in Gran Canaria over 11 years, and to analyse the outcomes of such rehabilitation.

Methods: The records of 2390 birds were analysed. Ten primary causes of morbidity were identified: trauma, metabolic/nutritional disorder, orphaned young birds, infectious/parasitic disease, crude oil, poisoning/intoxication, glue trapping, captivity, other causes, and unknown/undetermined. The rates of the final outcome categories (euthanasia E_r, unassisted mortality M_r, release R_r, and permanent captivity C_r), the time until death, and the length of stay were studied.

Results: The Eurasian Stone-curlew Burhinus oedicnemus was the species most frequently admitted (20.29%), followed by the Eurasian Blackbird Turdus merula (13.47%). The most frequent cause of morbidity was trauma (27.82%). The final outcomes of birds admitted alive were E_r= 16.69%, M_r= 26.53%, R_r= 54%, and C_r= 2.76%.

Conclusions: This survey provides useful information for the conservation and welfare of these bird species. The successful release rate achieved at the TWRC emphasizes the importance of wildlife rehabilitation centres for the conservation of birds.

09
Nov 17
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Acute phase proteins and markers of oxidative stress to assess the severity of the pulmonary hypertension in heartworm-infected dogs

Parasites and Vectors

Open AccessVolume 10, 9 November 2017, Article number 477

Carretón, E.a, Cerón, J.J.b, Martínez-Subiela, S.b, Tvarijonaviciute, A.b, Caro-Vadillo, A.c, Montoya-Alonso, J.A.a

^aFaculty of Veterinary Medicine, Research Institute of Biomedical and Health Sciences (IUIBS), University of Las Palmas de Gran Canaria, Trasmontaña s/n, Las Palmas, Gran Canaria, Arucas, Spain
^bInterdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Veterinary School, University of Murcia, Murcia, Spain
^cMedicina y Cirugía Animal, Faculty of Veterinary Medicine, University Complutense of Madrid, Madrid, Spain

Abstract

Background: Canine heartworm infection is characterized by pulmonary endarteritis and pulmonary hypertension (PH). The aim of the present study was to evaluate the relationship between PH with the concentrations of different positive (C-reactive protein [CRP] and haptoglobin [Hp]) and negative (albumin and paraoxonase-1 [PON-1]) acute phase proteins (APP), as well as the oxidative stress, by measuring glutathione peroxidase (GPx) and total antioxidant capacity (TAC) in 27 heartworm-infected dogs on Day 0 (diagnosis) and Day 120 (1 month after the last adulticide injection). Presence/absence of PH was determined by the Right Pulmonary Artery Distensibility (RPAD) Index. Results: On Day 0, 62.9% of the dogs showed PH. Concentrations of CRP and Hp were higher in dogs with PH, especially in dogs with moderate-severe PH (P < 0.005 and P < 0.05, respectively). Albumin and PON-1 concentrations were higher in dogs without PH (P < 0.05 for albumin). On Day 120, 59.2% of the dogs presented with PH; CRP decreased while Hp increased (P < 0.005 and P < 0.05, respectively). Also, albumin and PON-1 rose, especially in absence of PH. There were not significant changes in the serum values of GPx and TAC. Conclusions: CRP and Hp have a potential prognostic role in dogs with dirofilariasis because increases in positive APP correlated with presence and severity of PH. CRP decreased, but Hp persisted at an elevated level in dogs with PH 1 month after the end of the adulticide treatment. CRP and Hp could work as early biomarkers of PH and be useful to stage the disease and to monitor the evolution of the patient and indirectly evaluate the persistence of arterial damage after the parasites have been eliminated. Albumin and PON-1 also showed potential value as markers of PH, although further research is necessary to determine its utility.

Enlace al documento

09
Nov 17
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Cardiopulmonary and inflammatory biomarkers in heartworm disease

Parasites and Vectors

Open AccessVolume 10, 9 November 2017, Article number 534

Carretón, E, Morchón, R.b, Montoya-Alonso, J.A.

^aFaculty of Veterinary Medicine, Research Institute of Biomedical and Health Sciences (IUIBS), University of Las Palmas de Gran Canaria, Trasmontaña s/n, Arucas, Las Palmas, Spain
^bLaboratory of Parasitology, Faculty of Pharmacy, Institute of Biomedical Research of Salamanca(IBSAL), University of Salamanca, Salamanca, Spain

Abstract

In heartworm disease, several biomarkers of cardiopulmonary injury and inflammatory activity have been studied during the recent years. D-dimer is a fibrin degradation product present after a clot is degraded, which has been reported to provide support for the diagnosis of pulmonary thromboembolism in heartworm disease. Furthermore, concentrations increment with increased disease severity and during the adulticide treatment. This increase in concentration has proved to be valuable. Cardiac biomarkers troponin I, myoglobin and NT-proBNP demonstrated presence of myocardial injury and heart failure, especially in chronic infections, which in some cases, slightly improve after the adulticide treatment. An acute phase response in dogs with Dirofilaria immitis, characterized by variations of acute phase proteins (APP), has been reported, indicating inflammatory processes that could contribute to disease progression. Among them, C-reactive protein (CRP) increases according to the severity of the disease; and a strong correlation between pulmonary hypertension and CRP has been observed. In cats, little work has been done to ascertain the utility of these biomarkers in feline heartworm; the only published study in D. immitis-seropositive cats reported significantly higher concentrations in positive APP serum amyloid A, haptoglobin and ceruloplasmin.

Enlace al documento

04
Oct 17
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A Case of Congenital Supernumerary Teeth in an Ovine Dental Pad.

J Vet Dent. 2017 Dec;34(4):279-281. doi: 10.1177/0898756417734378. Epub 2017 Oct 4.

Corbera JA¹, Morales I¹, Martin S¹, Arencibia A², Gutierrez C¹.

Abstract

A rare case of congenital supernumerary teeth, also known as hyperdontia, observed in a healthy 8-month-old female ewe is presented. The congenital defect consisted of the presence of 2 incisor teeth embedded in the lateral areas of the dental pad. The anomaly was found during a routine physical examination and no other congenital abnormalities were found in the patient. No prior congenital abnormalities had been seen in the herd and the study of possible associated teratogenic factors was inconclusive. To the authors’ knowledge, this ovine odontogenic abnormality has not been described in the literature and appears to be an extraordinarily rare condition.

07
Jun 17
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Effect of plasma rich in growth factors on the early phase of healing of surgically severed Achilles tendon in sheep: histological study

Journal of Applied Animal Research 7 June 2017, Pages 1-8

Effect of plasma rich in growth factors on the early phase of healing of surgically severed Achilles tendon in sheep: histological study

Aguilar-García, D.^a, Fernández-Sarmiento, J.A.^a, Granados, M.D.M.^a, Morgaz, J.^a, Navarrete, R.^a, Carrillo, J.M.^b, Vilar, J.M.^c, Cugat, R.^d, Domínguez, J.M.^a

^aDepartment of Animal Medicine and Surgery, University of Córdoba, Córdoba, Spain
^bDepartment of Animal Medicine and Surgery, CEU Cardenal Herrera University, Valencia, Spain
^cDepartment of Animal Pathology, University Institute of Health and Biomedical Research, University of Las Palmas de Gran Canaria, Arucas, Spain
^dArtroscopia GC, Hospital Quirón, Barcelona, Spain

Older Posts

Author Archives: Grupo MVeIT

Jun 18

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Abstract

Abr 18

-

3-D computed tomography reconstruction: Another tool to teach anatomy in the veterinary colleges(Letter)

Mar 18

-

Posturography and dynamic pedobarography in lame dogs with elbow dysplasia and cranial cruciate ligament rupture(Article)

Abstract

Mar 18

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Effect of leukocyte-reduced platelet-rich plasma on osteoarthritis caused by cranial cruciate ligament rupture: A canine gait analysis model(Article)(Open Access)

Abstract

Feb 18

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The Canary Islands as a model of risk of pulmonary dirofilariasis in a hyperendemic area.

Abstract

Dic 17

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Nov 17

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Abstract

Nov 17

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Cardiopulmonary and inflammatory biomarkers in heartworm disease

Abstract

Oct 17

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Abstract

Jun 17

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Effect of plasma rich in growth factors on the early phase of healing of surgically severed Achilles tendon in sheep: histological study

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