Grupo MVeIT – Página 10 – Grupo de Investigación del Instituto Universitario de Investigaciones Biomédicas y Sanitarias (IUIBS) de la Universidad de Las Palmas de Gran Canaria.

08
Jun 12
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Evaluation of pulmonary function variables by using plethysmography in cats with respiratory disease associated to Dirofilaria immitis.

Veterinary Parasitology. Volume 187, Issue 1-2, 8 June 2012, Pages 254-258
García-Guasch, L., Caro-Vadillo, A., Manubens-Grau, J., Carretón, E., Morchón, R., Simón, F., Montoya-Alonso, J.A.

Abstract

Heartworm Associated Respiratory Disease (HARD) is a pulmonary syndrome that results from the vascular and parenchymal inflammatory response associated with the arrival and death of Dirofilaria immitis in the distal pulmonary arteries. Barometric whole-body plethysmography (BWBP) is a non-invasive pulmonary function test (PFT) that allows a dynamic study of breathing patterns and is useful to study airway disease and the response to different treatments. The aim of this prospective non-blinded study was to compare respiratory function variables between healthy cats and HARD cats (seropositive to D. immitis) by use of BWBP. Twenty-five healthy cats and six HARD cats were put into the plethysmograph chamber and different respiratory variables were measured. The results were analyzed and compared between the two groups of animals. There were significant differences for bronchoconstriction index variables Pause (P-value < 0.001) and enhanced pause (P-value < 0.001), minute volume (P-value < 0.05) and tidal volume (P-value < 0.05) between healthy and HARD cats. There were no significant differences in respiratory rate and inspiratory and expiratory times between both groups of animals. The results obtained in our study support that HARD cats show significant differences in pulmonary function variables obtained by BWBP due to an acute inflammatory response at bronchial, vascular and parenchymal level. This PFT could be a useful method to facilitate the diagnosis of pathological states of bronchoconstriction in HARD cats.

01
Jun 12
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Cross-sectional study on prevalence of Trypanosoma evansi infection in domestic ruminants in an endemic area of the Canary Islands (Spain)

Preventive Veterinary Medicine
Volume 105, Issue 1-2, 1 June 2012, Pages 144-148
Rodríguez, N.F., Tejedor-Junco, M.T. , González-Martín, M., Santana del Pino, A., Gutiérrez, C.

Abstract

Trypanosoma evansi is the most widely spread of the pathogenic African trypanosomes of animals. The disease (surra) was first diagnosed in the Canary Islands in a dromedary camel in 1997; thus, a control plan was implemented achieving the eventual eradication of T. evansi from most of the infected areas in the Archipelago. However, a little area remains still infected despite the use of the same control measures. To evaluate possible reservoirs in the area a representative sample of domestic ruminants was examined by serological, parasitological and molecular tests. Of a total of 1228 ruminants assessed, 61 (5%) were serologically positive (7 cattle, 21 goats, 33 sheep), but T. evansi could be demonstrated in none of them. According to FreeCalc assessment, cattle and goat populations would be free from disease; however, the results from sheep are not adequate to conclude that the population would be free from disease. As a conclusion, surveillance must be exercised on ruminant farms in the surroundings of the infected area in order to evaluate the possible extension of the disease and their potential role as reservoirs of T. evansi.

29
May 12
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Generation of single domain antibody fragments derived from camelids and generation of manifold constructs (Article)

Methods in Molecular Biology
Volume 907, 2012, Pages 145-176
Vincke, C., Gutiérrez, C., Wernery, U., Devoogdt, N., Hassanzadeh-Ghassabeh, G., Muyldermans, S.

Abstract

Immunizing a camelid (camels and llamas) with soluble, properly folded proteins raises an affinity-matured immune response in the unique camelid heavy-chain only antibodies (HCAbs). The peripheral blood lymphocytes of the immunized animal are used to clone the antigen-binding antibody fragment from the HCAbs in a phage display vector. A representative aliquot of the library of these antigen-binding fragments is used to retrieve single domain antigen-specific binders by successive rounds of panning. These single domain antibody fragments are cloned in tandem to generate manifold constructs (bivalent, biparatopic or bispecific constructs) to increase their functional affinity, to increase specificity, or to connect two independent antigen molecules.

12
Mar 12
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Dirofilariosis: Pautas de manejo clínico.

Autores: Montoya-Alonso JA y Carretón E:

«Dirofilariosis: Pautas de manejo clínico es un libro de consulta rápida que, de manera sencilla y con muchos esquemas e ilustraciones, aporta respuestas actualizadas sobre la enfermedad en el hombre y en varias especies animales susceptibles.”

Editorial (año): Multimédica. Barcelona, 2012

ISBN: 978-84-96334-44-0

Enlace a Multimédica Ediciones Veterinarias

12
Mar 12
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Manual de Electrocardiografía Clínica Canina 2ª Ed.

Autores: Ynaraja E y Montoya-Alonso JA:

El libro «Manual de electrocardiografía clínica» alcanza su segunda edición, pero sigue conservando el espíritu de manual de consulta rápida para la clínica diaria. En este manual se hace hincapié en la importancia del electrocardiograma como herramienta diagnóstica. Presenta una revisión exhaustiva de patologías cardiacas con sus correspondientes electrocardiogramas y dedica un capítulo a la descripción de la técnica diagnóstica.

Editorial (año): Servet Editorial. Zaragoza, 2012

ISBN: 978-84-92569-82-3

Enlace Web Grupo Asis

17
Feb 12
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Epidemiología clínica y molecular de la tripanosomosis animal por trypanosoma evansi en Canarias

Tesis Doctoral Dr. D. Noé Francisco Rodríguez González

Departamento de Patología Animal, Producción Animal, Ciencia y Tecnología de los Alimentos

Directores: Dr. D. Carlos Javier Gutiérrez Cabrera, Dra. Dña. María Teresa Tejedor Junco y Dra. Dña. Margarita Rosa González Martín

Los objetivos de este trabajo son: 1. Comparar las técnicas diagnósticas de concentración del hematocrito (MHCT) y de la reacción en cadena de la polimerasa (PCR) para detectar T.evansi en cabras inoculadas experimentalmente, de cara a su utilidad en el posterior estudio de campo sobre rumiantes. 2. Determinar el papel de los rumiantes domésticos en la epidemiología de T.evansi en la isla de Gran Canaria. 3. Determinar el papel de los équidos residentes o próximos a la zona afectada en la epidemiología de la enfermedad en Gran Canaria. 4. Determinar los vectores responsables de la transmisión de la enfermedad en Canarias. 5. Determinar el papel de los roedores silvestres en la epidemiología de T. evansi en la isla de Gran Canaria.

Fecha de lectura: 17/02/2012

Texto completo en ACCEDA

01
Feb 12
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Excretory/secretory antigens from Dirofilaria immitis adult worms interact with the host fibrinolytic system involving the vascular endothelium

Molecular and Biochemical Parasitology
Volume 181, Issue 2, February 2012, Pages 134-140
González-Miguel, J., Morchón, R., Mellado, I., Carretón, E., Montoya-Alonso, J.A., Simón, F.

Abstract

Dirofilaria immitis is the causative agent of canine and feline heartworm disease. The parasite can survive for long periods of time (7 years or more) in the circulatory system of immunocompetent reservoirs, producing usually a chronic inflammatory vascular disease. In addition, the simultaneous death of groups of adult worms can trigger an acute disease characterized by the exacerbation of inflammatory reactions and the emergence of serious thromboembolic events. In the context of the D. immitis/host relationships, the aim of this study was to investigate the interaction between the excretory/secretory antigens from D. immitis adult worms (DiES) and the fibrinolytic system of the host. Using an enzyme-linked immunosorbent assay we showed that DiES extract is able to bind plasminogen and generate plasmin, although this fact requires the presence of the tissue plasminogen activator (t-PA). Moreover, we established that DiES extract enhances t-PA expression in cultured vascular endothelial cells. Additionally, 10 plasminogen-binding proteins from DiES extract were identified by mass spectrometry (HSP60, actin-1/3, actin, actin 4, transglutaminase, GAPDH, Ov87, LOAG-14743, galectin and P22U). The data suggest that DiES antigens interact with the environment of the parasite regulating the activation of the fibrinolytic system of the host with involvement of the vascular endothelium in the process.

29
Ene 12
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Sperm viability of canine and caprine semen samples preserved in a dry shipper

Animal Reproduction Science
Volume 130, Issue 1-2, January 2012, Pages 105-110
Batista, M. , Santana, M., Niño, T., Alamo, D., Cabrera, F., González, F., Gracia, A.

Abstract

This study assessed the efficacy of a dry shipper to preserve canine and caprine semen samples. After equilibration, semen straws from six Majorera bucks and five dogs were frozen and stored in liquid nitrogen (LN). Thirty days after freezing, half of the frozen straws were transferred from LN to a dry shipper (DS). Then, thawing was performed at 1, 2, 3, 5 and 7 days and the percentages of motile spermatozoa, acrosome intact spermatozoa and abnormal spermatozoa were determined. The sperm motility (total and progressive) of canine semen samples preserved with DS was quite similar to those preserved in LN, and no significant differences were observed throughout the experimental period. In addition, no differences were observed in the number of abnormal spermatozoa (range: 13.2-19.0%) or intact acrosome (range 91.3-95%) between both storage protocols. Buck semen samples showed equivalent levels of progressive motility (between 50% and 60%) and intact acrosome membrane (around 70%) during the first 3 days of storage in both procedures; however, from the fifth day of storage onwards, a notable decrease in semen quality was observed in the samples preserved in DS, showing a dramatic fall in the semen viability after 7 days of preservation (12.3% and 36.8%, progressive fast spermatozoa and acrosome integrity, respectively). In dog samples, the present study confirmed that seminal quality did not show modifications for the preservation period (7 days), confirming the efficacy of the dry shipper to preserve frozen samples for a short time. However, under the circumstances reported in this study, the sperm quality of buck samples preserved in the dry shipper only held during the first 3 days of storage, and therefore, its practical application could be more limited.

01
Ene 12
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Anti-wolbachia surface protein antibodies are present in the urine of dogs naturally infected with dirofilaria immitis with circulating microfilariae but not in dogs with occult infections

Vector-Borne and Zoonotic Diseases
Volume 12, Issue 1, 1 January 2012, Pages 17-20
Morchón, R., Carretón, E., Grandi, G., González-Miguel, J., Montoya-Alonso, J.A., Simón, F., Genchi, C., Kramer, L.H.

Abstract

Heartworm infection (Dirofilaria immitis) can cause kidney damage due to the presence of circulating microfilariae (mf) that contribute to the production and deposit of immune complexes. It has been shown that mf are a major source of Wolbachia antigen during active infection. Here the authors compared urine samples from 19 naturally infected dogs with (mf+) and 12 without (mf-) microfilariae for the presence of proteinuria and anti-Wolbachia Surface Protein (-WSP) IgG in ELISA. Kidneys from 6 mf+ and 3 mf-dogs were also examined by anti-WSP immuno-histochemistry. All infected dogs showed proteinuria, but mf+ dogs had significantly higher values compared to mf-dogs. Mf+ dogs had optical density values for anti-WSP IgG consistently higher than established cut-off values and were significantly higher than values for mf-dogs. Kidneys from mf+ dogs showed Wolbachia+ mf in glomerular capillaries. Results strongly suggest that Wolbachia associated with circulating mf may contribute to immune-mediated kidney disease in dogs with heartworm infection.

01
Dic 11
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Proximal isovelocity surface area variability during systole in dogs with mitral valve prolapse

Journal of Veterinary Cardiology
Volume 13, Issue 4, December 2011, Pages 267-270
Paiva, R.M.P. , Garcia-Guasch, L., Manubens, J., Montoya-Alonso, J.A.

Abstract

The proximal isovelocity surface area (PISA) principles and methodology have been described in human and veterinary medicine with special emphasis given to the ease and speed for the quantification of mitral regurgitation. Although limitations have been described in both human and veterinary medicine, in the case of veterinary medicine, clinical examples have not been well defined and in some cases have not been individually presented. The objective of this paper is to illustrate in a qualitative manner the echocardiographic existence of the dynamic behavior and variability of mitral regurgitation in dogs with mitral valve prolapse, a factor to be taken into consideration as a limitation inherent to the PISA technique.

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