Author Archives: Grupo MVeIT

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Influence of different anaesthetic protocols over the sperm quality on the fresh, chilled (4°C) and frozen-thawed epididymal sperm samples in domestic dogs

Volume 51, Issue 5, 1 October 2016, Pages 758-765

Batista, M. ,Vilar, J., Rosario, I., Terradas, E.

Unit of Reproduction, Universitary Institute of Biomedical Research and Health, University of Las Palmas de Gran Canaria, Arucas, Las Palmas, Spain


This study assessed the influence of three different anaesthetic protocols on semen quality obtained from the epididymis. Sixty male dogs undergoing to routine sterilization were assigned to three anaesthetic protocols: thiopental group (TG, n = 20), propofol group (PG, n = 20) and ketamine–dexmedetomidine group (KDG, n = 20). Immediately after orchidectomy, the cauda epididymides and vas deferent ducts were isolated and then a retrograde flushing was performed to collect spermatozoa. In experiment 1, after the initial evaluation of the semen (sperm concentration, sperm motility and the percentages of live spermatozoa, abnormal spermatozoa and acrosome membrane integrity), semen samples were diluted in Tris-glucose-egg yolk extender and chilled for 48 hr, and the sperm motility was assessed at 6, 24 and 48 hr. In experiment 2, semen samples were diluted in Tris-glucose-egg yolk extender and chilled for 24 hr, and then samples were frozen in two extenders with different glycerol concentrations, to reach a final concentration of 50–100 × 106 spermatozoa ml−1, 20% egg yolk, 0.5% Equex and 4% and 5% glycerol, respectively. Mean values of total sperm concentration, sperm viability and the percentages of intact acrosome and abnormal spermatozoa were not significantly different between experimental groups, and therefore, the anaesthetic protocols assessed did not affect sperm parameters mentioned above. However, our study confirmed a detrimental effect of the use of thiopental (TG) over the total sperm motility (p < 0.05) and progressive sperm motility (p < 0.05) of the fresh and chilled epididymal sperm samples. The anaesthetic protocols including the application of propofol or ketamine–dexmedetomidine can be used to recover sperm in domestic canids without significant changes in sperm quality compared when semen is collected routinely and these techniques could be applicable to endangered wild canids.

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Characterization of nanobodies binding human fibrinogen selected by E. coli display 

Journal of Biotechnology
Volume 234, 20 September 2016, Pages 58-65
 Salema, V.a, López-Guajardo, A.a,  Gutierrez, C.b, Mencía, M.c,  Fernández, L.Á.a

Research Institute of Biomedical and Health Sciences, Veterinary Faculty, Universidad de Las Palmas de Gran Canaria (UPGC), Arucas, Las Palmas, Canary Islands, Spain
Centro de Biología Molecular “Severo Ochoa” (Consejo Superior de Investigaciones Científicas—Universidad Autónoma de Madrid), Campus UAM Cantoblanco, Madrid, Spain


Abnormal levels of fibrinogen (Fib) in blood plasma are associated with several pathological conditions and hence methods for its detection in blood and body fluids are essential. Nanobodies (Nbs) or (VHHs) are single domain antibodies derived from camelids with excellent biophysical and antigen-binding properties, showing great promise in diagnostics and therapy. In this work, we select and characterize high affinity Nbs binding human Fib employing an E. coli cell surface display system based on the fusion of an immune library of VHH domains with the β-domain of Intimin. Bacteria displaying high-affinity Nbs against Fib were selected using magnetic cell sorting (MACS). Specific binding of the selected clones to Fib was confirmed by flow cytometry of E. coli bacteria, as well as by enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR) with the purified Nbs. E. coli display also provided an excellent estimation of the affinity of the selected Nbs by flow cytometry analysis under equilibrium conditions, with equilibrium constant (KD) values very similar to those obtained by SPR analysis. Finally, pairwise epitope-scouting studies revealed that the selected Nbs bound distinct epitopes on Fib. The selected Nbs are promising diagnostic tools for determination of human Fib levels.

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Therapeutic doses of plasma rich in growth factors cannot provoke cancer by means of the IGF-1 pathway or inflammation in dogs

Vilar, J.M., Damiá, E., Rubio, M., Santana, A., Sopena, J., Ceron, J.,
Tvarijonaviciute, A., Cugat, R., Carrillo, J.M.


A potential relationship between an increased risk of cancer and increased levels of serum insulin-like growth factor-1 (IGF-1), and presence of cancer and high levels of C-reactive protein (CRP) has been previously reported. This study evaluated the influence of a single intramuscular injection of plasma rich in growth factors (PRGF) on serum concentrations of IGF-1 and CRP in dogs. Two groups of eight healthy beagles were injected with two different doses of PRGF in lumbar muscles. For each treatment, IGF-1 and CRP were analysed from blood samples obtained at baseline and the following three days post injection. No differences were found when IGF-1 and CRP were compared among times in the two protocols. Local application of PRGF at clinical doses did not cause significant changes in systemic concentrations of IGF-1 or detectable inflammation. © 2016 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group


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Three-dimensional time of flight magnetic resonance angiography of the heart and associated vessels in a cat

Journal of Veterinary Cardiology,Volume 18, Issue 4, December 2016, Pages 413–417

A. Arencibia, DVM, PhD, , , J.A. Corbera, DVM, PhD, G. Ramírez, DVM, PhD, S. Contreras, DVM, M. Morales, DVM, PhD, J.R. Jaber, DVM, PhD, J. Orós, DVM, PhD, J.M. Vázquez, DVM, PhD.


The aim of this study was to describe the normal magnetic resonance angiography (MRA) of the heart and associated vessels in a mature female cat using a 1.5-Tesla magnet. Non-contrast enhanced MRA was performed using a three-dimensional time of flight (TOF) sequence in parasagittal and dorsal aspects. Relevant cardiac and vascular structures were labelled on three-dimensional Time of flight images. Time of flight imaging showed details of the heart cavities and vessels lumen due to the high signal intensity of fast-flowing blood compared with bones, muscles, and lungs, which appeared with low signal intensity. Three-dimensional TOF sequences provided adequate anatomical details of the heart and good differentiation of the vascular structures that could be used for interpretation of cardiac images and to assist in future MRA studies.

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Relative echogenicity of tendons and ligaments of the palmar metacarpal region in foals from birth to 4 months of age: A longitudinal study

Volume 11, Issue 7, 1 July 2016,

Spinella, G.a , Britti, D.b, Loprete, G.b, Musella, V.b, Romagnoli, N.a, Vilar, J.M.c, Valentini, S.a

a Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia (BO), Italy
b Department of Health Sciences, University Magna Graecia of Catanzaro, Germaneto (CZ), Italy
c Department of Animal Pathology, Faculty of Veterinary Medicine, University of Las Palmas de Gran Canaria, Trasmontaña S/N, Arucas, Las Palmas, Spain
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The objective of this study was to evaluate relative echogenicity of superficial and deep digital flexor tendons, the accessory ligament of the deep digital flexor tendon and interosseous muscle of the metacarpal region in foals ages 1 week to 4 months; and assess the association between echogenicity and sex or side/laterality. Seven Standardbred trotter foals were examined. Right and left metacarpal regions (palmar surface) were ultrasonographically investigated, and four regions of interest were assessed. A significant increase in echogenicity was seen in superficial and deep digital flexor tendons, accessory ligament of deep digital flexor tendon, and interosseous muscle during growth from 1 week to 4 months of age. Echogenicity of examined tendons and ligaments was not influenced by gender nor laterality. Reference values for tendon and ligament echogenicity could function as a tool to discriminate between physiological and abnormal conditions such as congenital contractural conditions.

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Polymorphism in ion channel genes of Dirofilaria immitis: Relevant knowledge for future anthelmintic drug design

Institute of Parasitology, McGill University, 21111 Lakeshore Road, Sainte-Anne-de-Bellevue, QC H9X 3V9, Canada
Research Institute of Biomedical and Health Sciences (IUIBS), Faculty of Veterinary, University of Las Palmas de Gran Canaria, Las Palmas de Gran Canaria, Spain
Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada


. Dirofilaria immitis, a filarial parasite, causes cardiopulmonary dirofilariasis in dogs, cats and wild canids. The macrocyclic lactone (ML) class of drugs has been used to prevent heartworm infection. There is confirmed ML resistance in . D. immitis and thus there is an urgent need to find new anthelmintics that could prevent and/or control the disease. Targeting ion channels of . D. immitis for drug design has obvious advantages. These channels, present in the nematode nervous system, control movement, feeding, mating and respond to environmental cues which are necessary for survival of the parasite. Any new drug that targets these ion channels is likely to have a motility phenotype and should act to clear the worms from the host. Many of the successful anthelmintics in the past have targeted these ion channels and receptors. Knowledge about genetic variability of the ion channel and receptor genes should be useful information for drug design as receptor polymorphism may affect responses to a drug. Such information may also be useful for anticipation of possible resistance development. A total of 224 ion channel genes/subunits have been identified in the genome of . D. immitis. Whole genome sequencing data of parasites from eight different geographical locations, four from ML-susceptible populations and the other four from ML-loss of efficacy (LOE) populations, were used for polymorphism analysis. We identified 1762 single nucleotide polymorphic (SNP) sites (1508 intronic and 126 exonic) in these 224 ion channel genes/subunits with an overall polymorphic rate of 0.18%. Of the SNPs found in the exon regions, 129 of them caused a non-synonymous type of polymorphism. Fourteen of the exonic SNPs caused a change in predicted secondary structure. A few of the SNPs identified may have an effect on gene expression, function of the protein and resistance selection processes.


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Causes of Admission for Raptors to the Tafira Wildlife Rehabilitation Center, Gran Canaria Island, Spain: 2003-2013

Journal of Wildlife Disease, 2016, 52:3, 647-652

Natalia Montesdeoca, Pascual Calabuig , Juan A. Corbera, and Jorge Orós.


We report the causes of morbidity of 2,458 free-living raptors admitted to the Tafira Wildlife Rehabilitation Center on Gran Canaria Island, Spain, during 2003–2013. The seasonal cumulative incidences were investigated while considering estimates of the wild populations in the region. These methods were used as a more accurate approach to assess the potential ecologic impact of different causes of morbidity. The most frequently admitted species were the Eurasian Kestrel (Falco tinnunculus; 53.0%), the Eurasian Long-eared Owl (Asio otus canariensis; 28.1%), the Canary Islands Common Buzzard (Buteo buteo insularum; 8.0%), and the Eurasian Barn Owl (Tyto alba; 4.4%). The most frequent causes of admission were trauma (33.8%), orphaned-young birds (21.7%), unknown (18.4%), and metabolic/nutritional disease (11.1%). Local morbidity caused by glue trapping and entanglement in burr bristlegrass (Setaria adhaerens) had prevalences of 5.0% and 1.8%, respectively. The highest number of admissions during the breeding and nonbreeding seasons was observed for the Eurasian Barn Owl and the Barbary Falcon (Falco pelegrinoides), respectively, mainly due to trauma of unknown origin.


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Influence of parasitism in dogs on their serum levels of persistent organochlorine compounds and polycyclic aromatic hydrocarbon

 Sci Total Environ. 2016 Apr 17;562:128-135. doi: 10.1016/j.scitotenv.2016.03.204.

Henríquez-Hernández, L.A.abCarretón, E.cCamacho, M.abMontoya-Alonso, J.A.c,Boada, L.D.ab,Valerón, P.F.ab,Cordón, Y.F.c,Almeida-González, M.ab,Zumbado, M.ab,Luzardo, O.P.ab 

Toxicology Unit, Research Institute of Biomedical and Health Sciences (IUIBS), University of Las Palmas de Gran Canaria, Cabrera Felipe s/n, Las Palmas de Gran Canaria, Spain
Spanish Biomedical Research Centre in Physiopathology of Obesity and Nutrition (CIBERObn), Paseo Blas Cabrera Felipe s/n, Las Palmas de Gran Canaria, Spain
Internal Medicine Service, Faculty of Veterinary Medicine, Research Institute of Biomedical and Health Sciences (IUIBS), University of Las Palmas de Gran Canaria, Paseo Blas Cabrera Felipe s/n, Las Palmas de Gran Canaria, Spain


Persistent organochlorine pollutants (POPs) are toxic chemicals, which accumulate in humans and animals, as only few species have the capability of eliminating them. However, some authors have pointed to the possibility that certain species of invertebrates (i.e. nematodes) could metabolize this type of compounds. As certain species of nematodes act as parasites of vertebrates, this research was designed to explore the influence of some of the most common parasites of the dogs in their serum levels of 56 common POPs. The study included three groups of dogs (n = 64), which were prospectively recruited in the island of Gran Canaria (Canary Islands, Spain): a) control animals, non-parasitized (serologically tested negative, n = 24); b) dogs tested positive for intestinal parasites and negative for other parasites (n = 24); and c) dogs tested positive for heartworm disease (Dirofilaria immitis) and negative for other parasites (n = 16). The presence of Dirofilaria immitis was strongly associated with lower serum levels of a wide range of pollutant in their hosts (PCB congeners 28, 52, 118, 138, 153, and 180; hexachlorobenzene, lindane, aldrin, dieldrin, anthracene and pyrene). We also found an inverse association between the hosts’ serum levels of PCBs and intestinal parasites. We did not find any association with DDT or its metabolites, but this might be explained by the recently suggested ability of dogs for the efficient metabolization of these compounds. According to the results of this study certain forms of parasitism would reduce the bioavailability of the major classes of POPs in dogs. However, further studies are needed to elucidate whether this phenomenon is due to a competence between parasites and hosts or could respond to a possible capability of parasitic nematodes for the metabolization of these POPs. © 2016 Elsevier B.V.

Author keywords

Dirofilaria immitis; Dogs; Organochlorine pesticides; Parasites; Polychlorinated biphenyls; Polycyclic aromatic hydrocarbons

Indexed keywords

Engineering controlled terms: Animals; Aromatic compounds; Aromatic hydrocarbons; Biochemistry; Body fluids; Chlorine compounds; Disease control; Hydrocarbons; Insecticides; Pollution; Polychlorinated biphenyls; Polycyclic aromatic hydrocarbons

Dirofilaria immitis; Dogs; Hexachlorobenzene; Intestinal parasites; Organochlorine pesticides; Parasites; Persistent organochlorine; Persistent organochlorine pollutants

Engineering main heading: Organic pollutants

Species Index: Animalia; Canis familiaris; Dirofilaria immitis; Invertebrata; Nematoda; Vertebrata

ISSN: 00489697 CODEN: STEVASource Type: Journal Original language: English
DOI: 10.1016/j.scitotenv.2016.03.204 Document Type: Article
Publisher: Elsevier

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Manual de Enfermedades Respiratorias en Animales de Compañía

Montoya-Alonso JA y Garcia-Guasch L

El principal objetivo de este manual es proporcionar una información práctica, comprensiva y razonable, y a su vez actualizada de las principales enfermedades respiratorias del perro y gato y de los nuevos animales de compañía (NAC).

Editorial (año): Multimédica Ediciones Veterinaria, Barcelona, 2016

ISBN: 978-84-96344-61-7


Enlace a editorial Multimédica

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Distribución de la enfermedad del gusanos del corazón en España y Portugal

Montoya-Alonso JA y Carretón E

ISBN: 978-84-608-4896-7

Editorial (año): Servicio de Medicina Veterinaria de la ULPGC. Las Palmas de Gran Canaria, 2016