Rectal pre-treatment with ozonized oxygen (O3) aggravates clinic status in septic rats treated with amoxicillin/clavulanate
Enfermedades Infecciosas y Microbiologia Clinica Monografias. Volume 33, Issue 7, 2015, Pages 469-475
Martín-Barrasa, J.L. , Cordovez, C.M.b De Los Monteros Y Zayas, A.E., D Santa Ana, M.C.J., Varas, B.C., Thomas, P.H., Benitez, A.B., Montoya-Alonso, J.A., García-Bello, M., Campelo, F.A.f Tejedor-Junco, M.T.
Abstract
Introduction: Despite the advanced antibiotic therapies, sepsis continues being a clinical entity with high morbidity and mortality. The ozone/oxygen mixture (O3/O2) has been reported to exhibit positive effects on immunity. The aim of our study was to analyze whether (O3/O2) combined with amoxicillin/ clavulanate has any influence on the morbidity and mortality of septic rats. Methods: We used 48 Sprague-Dawley rats randomly allocated to 6 groups (n = 8): healthy (C), septic (I), healthy + ozone therapy (O3), septic + amoxicillin/clavulanate (AMC), septic + amoxicillin/clavulanate + ozone therapy (AMC/O3) and septic + ozone therapy (I/O3). O3/O2 was administered rectally at increasing O3concentrations during 10 days prior to the onset of sepsis model (intraperitoneally injection of fecal material) or saline administration in healthy control rats. Later (post-inoculation), 3 days per week, O3was also administered. Vital signs were recorded, and microbiological, hematological and histopathological studies were performed. Results: The number of surviving animal/total was higher in AMC (8/8) than in AMC/O3 (4/8) p = 0.077. The percentage of surviving animals with pneumonia was higher in AMC/O3 than in AMC (100% vs 37.5%). In dead animals, AMC/O3 rats had a significantly higher percentage of lesions: Cardiac lesions, pulmonary hemorrhages and pleuritis (100%) and serositis/peritonitis (75%). Only Escherichia coli (2 different biotypes) was isolated from blood and/or peritoneal fluid from all infected groups. A significant decrease in the percentage of band neutrophils from the surviviors belonging to AMC/O3 vs AMC was observed (p 0.05). Conclusion: Rectal pre-treatment with O3/O2 aggravates clinic status in septic rats treated with amoxicillin/ clavulanate.
